Journal: medRxiv

Article Title: An accurate and rapid Real-time PCR approach for human Monkeypox virus diagnosis

doi: 10.1101/2022.06.23.22276033

Figure Lengend Snippet: Δ Rn vs cycle representation of the qPCR performed on the MIC cycler. MPXV PCR amplification curves from successive 10-fold serial dilutions of a positive sample with Ct values ranging from 15.3 to 22.3.

Article Snippet: PCR amplification was conducted on Mic qPCR (Bio Molecular systems) and AB™7500 Fast Real-Time PCR system (Applied Biosystems, USA) devices.

Techniques: Amplification

Journal: medRxiv

Article Title: An accurate and rapid Real-time PCR approach for human Monkeypox virus diagnosis

doi: 10.1101/2022.06.23.22276033

Figure Lengend Snippet: Δ Rn vs cycle representation of the qPCR performed on the QuantStudio™7 Flex Real-Time PCR System. MPXV PCR amplification curves from successive 5-fold serial dilutions of a positive sample with Ct values ranging from 16.5 to 37.3.

Article Snippet: PCR amplification was conducted on Mic qPCR (Bio Molecular systems) and AB™7500 Fast Real-Time PCR system (Applied Biosystems, USA) devices.

Techniques: Real-time Polymerase Chain Reaction, Amplification

Journal: Scientific Reports

Article Title: A plasmonic gold nanofilm-based microfluidic chip for rapid and inexpensive droplet-based photonic PCR

doi: 10.1038/s41598-021-02535-1

Figure Lengend Snippet: ( a ) Schematic illustration of the fundamental principle of plasmonic photothermal light-to-heat conversion for droplet-based photonic PCR (dpPCR): when a light is turned on, fast heating of sample droplets can be achieved by fast heat diffusion of hot electrons throughout the AuNF. When a light is turned off, the heat dissipation through the AuNF can occur, leading to the cooling of the heated droplets. After thermal cycling, the success of the amplification can be identified by analyzing the fluorescence intensity of droplets. ( b ) Circuit diagram of the plasmonic photothermal cycler. ( c ) An enlarged section of ( b ), indicating the position of the LED, heatsink, chips, and thermocouple.

Article Snippet: To assess the functionality of the plasmonic photothermal cycler, a PCR experiment was performed using a commercial PCR thermal cycler (Mic qPCR cycler, Bio Molecular Systems, Australia).

Techniques: Diffusion-based Assay, Amplification, Fluorescence

Journal: Scientific Reports

Article Title: A plasmonic gold nanofilm-based microfluidic chip for rapid and inexpensive droplet-based photonic PCR

doi: 10.1038/s41598-021-02535-1

Figure Lengend Snippet: ( a ) Schematic depiction of the photonic PCR thermal cycling, consisting of three successive temperatures for denaturation, annealing, and extension, using the Au nanofilm (AuNF) excited by the LEDs. Light that encounters AuNF (I 0 and I 1 ) can be reflected (R 0 and R 1 ), transmitted (T 0 and T 1 ), or absorbed (A 0 and A 1 ). ( b ) Comparison of temperature profiles of the parallel reaction chamber (PRC) between position P and position M to ensure that the temperature ramping rate of both chambers is the same. Inset shows a schematic of positions P and M. ( c ) Comparison of heating and cooling rates of the sample using the constructed chip with (stable bonding) and without (unstable bonding) tape layer.

Article Snippet: To assess the functionality of the plasmonic photothermal cycler, a PCR experiment was performed using a commercial PCR thermal cycler (Mic qPCR cycler, Bio Molecular Systems, Australia).

Techniques: Comparison, Construct

Journal: Scientific Reports

Article Title: A plasmonic gold nanofilm-based microfluidic chip for rapid and inexpensive droplet-based photonic PCR

doi: 10.1038/s41598-021-02535-1

Figure Lengend Snippet: ( a ) Temperature profiles of the single-sided plasmonic heating mechanism (ssPHM) and the dual-sided plasmonic heating mechanism (dsPHM) for the blue light. ( b ) Temperature profiles of the sample in the ssPHM and the dsPHM for denaturation-to-annealing ramp and vice versa (30 PCR thermal cycles, 95 °C for 0 s and 60 °C for 1 s). ( c ) Heating and cooling rates obtained from the dsPHM thermal cycling (average heating and cooling rates of 7.37 ± 0.27 °C/s and 1.91 ± 0.03 °C/s, respectively). Solid lines indicate average values. Dashed lines are placed one standard deviation away from averages.

Article Snippet: To assess the functionality of the plasmonic photothermal cycler, a PCR experiment was performed using a commercial PCR thermal cycler (Mic qPCR cycler, Bio Molecular Systems, Australia).

Techniques: Standard Deviation

Journal: Scientific Reports

Article Title: A plasmonic gold nanofilm-based microfluidic chip for rapid and inexpensive droplet-based photonic PCR

doi: 10.1038/s41598-021-02535-1

Figure Lengend Snippet: Illustration of the plasmonic photothermal cycling results with the dsPHM. ( a ) PCR thermal profile of a complete PCR reaction. The thermocycling program settings are 95 °C pre-denaturation for 8 min followed by 30 cycles of 95 °C (denaturation), 60 °C (annealing), and 72 °C (extension) for 15 s, 20 s, and 20 s, respectively. ( b ) Evaluation of temperature accuracy and stability. The average values with standard deviation at 95 °C, 60 °C and 72 °C are 94.99 ± 0.41 °C, 60.02 ± 0.37 °C and 72.01 ± 0.39 °C, respectively.

Article Snippet: To assess the functionality of the plasmonic photothermal cycler, a PCR experiment was performed using a commercial PCR thermal cycler (Mic qPCR cycler, Bio Molecular Systems, Australia).

Techniques: Standard Deviation